The main purpose of the present study was to investigate psychometric properties of the Questionnaire of Teacher Interaction (QTI, Lourdusamy & Khine, 2001) among Iranian teachers. In this correlation study, 200 teachers (100 males, 100 female) completed the Questionnaire of Teacher Interaction (QTI). The confirmatory factor analysis method and internal consistency were used to compute the QTI's factorial validity and reliability, respectively. The results of confirmatory factor analysis, based AMOS software, indicated that multidimensional structure of the QTI consisted leadership, helpful/friendly behavior, understanding, student responsibility and freedom, uncertain, dissatisfied, admonishing and strict behavior had acceptable fit to data in the Iranian teacher’ s sample. Internal consistencies for the scales of Questionnaire of Teacher Interaction ranged from 0/55 to 0/70. In sum, these findings provide evidence for the validity and reliability of the QTI as an instrument to measure model of interpersonal relation behavior among Iranian teachers.