Shirazi thyme is herbaceous perennial belonging to the mint family (Lamiaceae), it is extensively grown in Pakistan, Afghanistan and Iran, since both the flavor of foods and brewing is widely used. The research as a randomized complete design with three replications and the effects of gamma irradiation was investigated on the microbial load of Shirazi thyme (Zataria multiflora). Shirazi thyme leaves were irradiated at dosages 3, 7, 10 and 15 KGy by a Co60 source then (microbial load) total count, total coliform and mold and yeast of the samples were studied. Identification and analysis of essential oil compounds by hydro-distillation and analyzed by GC and GC/MS. The result showed that gamma irradiation decrease microbial load of Shirazi thyme samples. The lowest microbial load was detected in samples treated with 15 KGy radiations. According to The results of the analysis of essential oils, Carvacrol, Thymol and p-Cymene, Was determined the major component of essential oil of Shirazi thyme. The results showed that irradiation with gamma radiation, reduced the carvacrol, increased p-Cymene and g-Terpinene, Furthermore, the formed of component of the Camphene and (E)-citral. Dose of 10 KGy formated the a-thujen.