JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)
Year:2014 | Volume:32 | Issue:293|Papers Count:7

Background: The goal of this study was to evaluate the effect of high and low molecular weight of protein fraction obtained from Lactobacillus casei and L. paracasei cell wall on K562 cell line.Methods: Bacteria cultured in special medium and incubated in anaerobic condition and washed with PBS and then sonicated and centrifuged for cell wall separation. Protein precipitation was conducted with trichloroacetc acid (72%) and sodium de oxalate (15%). Then different concentrations (500, 1000, 2000, 3000 and 4000 mg/ml) of cell wall proteins were prepared in cell culture medium for each bacteria separately. In vitro anti cancer properties of both bacteria proteins were assayed in 12, 24, 48 and 72 hour with a colorimetric method (MTT) with 10000 cells per well.Findings: Results showed that high and low molecular weight proteins of both bacteria could significant (p=0.046) inhibit the growth of K562.Conclusion: Based on the results we conclude that high molecular weight of both probiotic bacteria significantly could inhibit the growth of K562 than the low molecular protein fractions. Also, protein fraction of L. paracasei significantly had anti cancer properties than protein fraction of L. casei.

Background: The purpose of this study was to determine the Epidemiological characteristics and Incidence rate of brucellosis from 1999-2012 in the Tiran-Karvan Township in Isfahan province.Methods: The present study is a cross-sectional study that performed on all patients with diagnosis brucellosis in public and private sectors in the Tiran-karvan Township from 1999 to 2012, that for which brucellosis form completed. For analysis of data, we use SPSS19.donsity incidence rate calculated and reported based on total person-years in study duration.Findings: Average density incidence rate in a 14-year period was 52.96 per 100000 person-years. In urban area this rate was 5.67 and in rural area 52.54. The highest incidence in people who are employed in agriculture and animal husbandry and the least level was for students. So, this rate for agricultures 10.19, agriculture- Rancher 12, housewife andRancher16, students6.83andtheother occupationsis13 per 100000 person-years.Conclusion: Average incidence of brucellosis in the Tiran-karvan was higher than province average and the trend of its incidence was rising. However, the disease is much more common in rural than urban areas.

Background: Gestational weight gain and BMI play an important role in the outcome of pregnancy. Weight gain during pregnancy based on maternal pre-pregnancy BMI is determined. In This study was evaluated the relationship between body mass index and weight gain of pregnancy with maternal-fetal outcomes.Methods: In this study were enrolled 476 healthy pregnant women with singleton fetus and were divided based on body mass index and weight gain of pregnancy. Information on maternal age, sociodemographic status, parity, early pregnancy weight, gestational weight gain, maternal height, previous history of preterm delivery, nausea and vomiting were collected by a medical-midwifery questionnaire. Phases of labor (delivery), gestational age, mode of delivery, neonatal characteristics and postpartum bleeding was recorded in the delivery information form. After statistical analysis, the relation between body mass index and weight gain during pregnancy, maternal and neonatal outcomes were determined.Findings: Pregnancy outcomes were better in women with normal weight gain. In women, low weight and low weight gain during pregnancy, were more low birth weight. Also, women with overweight and gestational weight gain were exposed higher rates of cesarean delivery and postpartum hemorrhage. Significant differences was found between maternal body mass index with induction of labor, fetal malposition, mode of delivery, birth weight, nausea, vomiting, and weight gain during pregnancy. Women with adequate weight gain had an appropriate outcome in relation to weight, length, head and chest circumference of newborns and methods of delivery and postpartum hemorrhage.Conclusion: Our findings indicate that women with abnormal body mass index in early pregnancy or inappropriate weight gain during pregnancy should have special care to prevent complications of pregnancy and childbirth and protect the health of mother and child.

Background: In recent decades relation between ROS concentration and semen quality was noted. Saffron has traditionally been not only considered as a food additive but also as a medicinal herb, which has a good antioxidant properties. Vitamin E is considered as a endogenous and supplementary antioxidant which can protects the cell. The aim of this study was to evaluate the protection influence of saffron and vitamin E on sperm DNA against acid.Methods: Thirty adult male Wistar rats divided into saffron (n=10), vitamin E (n=10) and control (n=10) groups randomly. Rats received saffron (100 mg/kg/day), vitamin E (100 mg/kg/day) and distilled water (0.5 cc/day) in saffron, vitamin E and control groups respectively. After 60 days, cauda epididymis dissected and sperm were used for analysis of sperm chromatin susceptibility to acid denaturation by acridine orange (AO) staining. AO staining was carried out with or without acid detergent incubation.Findings: Our results purposed that saffron and vitamin E can decrease sperm DNA damage against acid.Conclusion: Saffron and vitamin E protect DNA against denaturation probably because of their powerful antioxidant properties.

Background: Cystinuria, one of the first inborn errors of metabolism, is recognized by hyperexcretion of cystine, lysine, ornithine arginine and into urine. So far, two genes associated with cystinuria have been identified: SLC3A1 (2p16.3) that encodes the heavy subunit rBAT of the renal b0,+ transporter and SLC7A9 (19q13.1), which encodes the light subunit b0,+AT. Patients with type A cystinuria have two SLC3A1 mutations, whereas patients with type B cystinuria have two SLC7A9 mutations and finally patients with type AB have one mutation in each gene. Considering the population-specific distribution of mutations in disease, limited studies on the genetic bases of the cystinuria have been done in Middle East. This research presents the results of mutation analysis on patients with cystinuria in Iran.Methods: Thirty unrelated cystinuria patients operated to remove kidney stones were screened by urologyst .The patients were analyzed for mutation using ARMS-PCR and RFLP-PCR methods. Findings: We found some variations including missense mutations, polymorphism and intron variant, but the most frequent mutations, M467T and also T216M and R333W, were not detected in our patients.Conclusion: Our research may confirm the ethnic distribution of mutations in cystinuria and hopefully this study will expand our concept of the genetic basis of cystinuria in Iranian patients.