In order to study the effect of Iranian zeolite (clinoptilolite) in manure on the yield, elements concentration of leaf and grain, amount of chlorophyll and leaf area index of sunflower under different irrigation regimes, an experimental was conducted in research farm of Tarbiat Modares University, college of agriculture, Tehran, Iran in 2006-2007. The experimental design was a spilt plot with four replications in form complete randomized block. The treatments composed of irrigation regimes at two levels: irrigation after using 35% available water (W1) and irrigation after using 70% available water (W2) as main plots and different treatments of manure at five levels: 130 kg N ha-1 by urea (chemical fertilizer) (F1), 80 kg N ha-1 by urea and 50 kg N ha-1 by manure (F2), 80 kg N ha-1 by urea and 50 kg N ha-1 by manure in combination with 5% weight of manure, natural zeolite (F3); 80 kg N ha-1 by urea and 50 kg N ha-1 by manure in combination with 10% weight of manure, natural zeolite (F4); 80 kg N ha-1 by urea and 50 kg N ha-1 by manure in combination with 15% weight of manure, natural zeolite (F5) as subplots. The result showed that the effect of irrigation regimes on grain and biological yield, chlorophyll of leaf in seed filling stage and leaf area index in flowering stage were significant, but elements concentration in leaf and grain were not affected by irrigation regimes. The result also showed that grain and biological yield, nitrogen concentration of leaf and grain, chlorophyll of leaf and leaf area index in flowering stage were affected significantly by fertilizers. From (F5) highest grain yield, biological yield and nitrogen concentration of grain and from (F1) highest nitrogen concentration and chlorophyll of leaf in flowering stage were obtained. Interaction of two factors had significant effect on biological yield, chlorophyll of leaf in seed filling stage, leaf area index in flowering stage and water use efficiency (WUE). Finally, regarding to result of this experiment and significant effect of application of Iranian natural zeolite for decreasing of using chemical fertilizers and avoiding of unsuitable effects of these chemical materials on environment, treatment 80 kg N ha-1 by urea and 50 kg N ha-1 by manure in combination with 15% weight of manure, natural zeolite (F5) under the first irrigation regime (WI F5), can be suggested as the best treatment.