Sedentariness and inactivity exposes men to various diseases and premature death. In industrialized societies, most people do not have physical activity. This way of life causes that the number of obese people, inactive people and the risk of liver diseases increase. The aim of this study was reviewing the effect of two weeks detraining after 8 weeks of aerobic training on alanin aminotransferase and aspartate aminotransferase of inactive men. In this clinical trial,18 inactive men selected and according to aerobic capacity divided to two groups of 9 subject with mean and standard deviation, of age (37.64 ± 4.7, 35.60 ± 2.54 years), weight (79.81 ± 7.66, 84.00 ± 9.79 kg) and height (173.91 ± 4.32, 175.55 ± 5.10 cm) respectively for experimental and control groups. experimental group ran on treadmill for eight weeks, three days a week and 30 minutes each session (the first week started with 50% of heart rate reserve and reached during the eight weeks to 70% of heart rate reserve), Then, training stoped for two weeks. 24 hours before the start of training, 24 hours after the last training session and two weeks after detraining period, Blood was collected from all subjects. Kolmogorov-Smirnov test, analysis of variance with repeated measures along with tukey’s post hoc test used for analysis of data(p=0.005). Results showed that detraining caused a significant increase of aspartate aminotransferase (p=0.005) but has no significant effect on decrease of alanine aminotransferase (p=0. 568). It can be concluded that eight weeks of selected aerobic training caused changes in the level of liver enzymes and also two weeks of detraining cause changes in the level of liver enzymes.