Year:2007 | Volume:19 | Issue:1 (74 IN ANIMAL AND FISHERIES SCIENCES)|Papers Count:32

One of ways for conservation of sturgeon fish is performance of artificial propagation. In order to improve of incubators is important. At present for incubation of sturgeon eggs utilize from Yushchenko (Y) incubator. In 2005, Azarakhsh (A) incubator was made in Iran, for comparison of efficiency with Yushchenko incubator on four Acipenser persicus brood stocks with emphasis on evaluation parameter of fertilization percentage in blastula stage( A=96.2 , Y=90.2 ), the mortality percentage of larva before (A=5.4, Y=10.6 ),and after ( A=8.6, Y=16.4 ) of active feeding and survival (A=86, Y=73) were occurred. In this survey the Azarakhsh incubator was better than Yshchenko incubator these issues demonstrated. The advantage of Azarakhsh incubator includes in less noise in activity and get out automatically from incubator vessel.

This study was conducted to survey the effect of chemisorption compounds on decreasing of aflatoxin M1 from raw milk. Contaminated milk samples to aflatoxin M1 were obtained from two dairy cows that were in mid lactation and were fed with contaminated diets by aflatoxin B1 for a 20- days period. Chemisorption compounds used in this experiment were kinds of bentonite and hydrated sodium calcium alumino – silicate (HSCAS). The samples of contaminated milk (87.4 ng/lit aflatoxin M1) were tested in two levels (%0. 5 and %2) of these compounds in room temperature (250c, 30 minutes). Toxin decreasing, milk composition, milk pH and the residues of some elements (Al and Si) that may be present after treatment were surveyed. The kind of chemisorption compounds, applied level and their interaction had noticeable effects for decreasing of milk aflatoxin (p< 0.01). Milk aflatoxin was decreased more than % 90 by bentonite B1, but decreasing of aflatoxin M1 by HSCAS and bentonite X was about % 77 and % 71 respectively. Milk composition consist of protein, fat, lactose, solid non fat and total solid were affected by addition of some adsorbents (p<0.05), although values of increasing or decreasing of milk components were negligible. As a result, there were no residues of aluminium, but a low level of silicium was left in the samples that were treated with chemisorptions compounds. This study showed that utilization of chemisorptions compounds had high efficiency for detoxification of aflatoxin M1 from milk. Because in this method does not usually involve degrading the toxin, milk may be free from toxin degradation products and is safe for consumption. In addition, the added material may be easily separated from milk after the substance adsorbs the toxin. Thus, this method should be developed by further researches for determining effects of these compounds on functional properties of milk.

The Siahdarvishan river is one of the important rivers entering to Anzali Lagoon and it has an important role in restoration of Caspian Sea migratory fishes annually. Main purposes of the study were: Determination of fish diversity, spatial and temporal distribution and present role of the river in restoration of migratory fishes. Studied fishes have been sampled in 8 stations from Upstream (Toolamshahr) as far as 20 km to downstream (Lakesaar) from winter 1998 until fall 2000. Electroschoker, cast net and beach seine were used to catch them. Results showed that 30 species and subspecies fish belonging to 11 families: Lampreys (Petromyzontidae), Sturgeons (Acipenseridae), Shads (Clupeidae), minnows (Cyprinidae), catfish (Siluridae), loaches (Cobitidae), pikes (Esocidae), salmon (Salmonidae), Mosquito fishes (Poeciliidae), Perches (Percidae) and gobies (Gobiidae) exist in the river so that Cyprinidae with 20 species and maximum abundance (95.9%) is prevalent and others had one represent. Capoeta capoeta gracilis was prevalent in total seasons and in stations 1, 3, 4 and 6, too and Carassius auratus gibelio in stations 2, 5, 7 and 8. In addition, C. capoeta gracilis, C. auratus gibelio and Chalcalburnus chalcoides with abundance 36.61, 26.17 and 9.94 % of total number of caught fishes in the study were respectively abundant. In the study was observed the least diversity (13 species) in upstream station (S 1) and the most diversity (27 species) in downstream station (S 8). The mentioned species with Alburnoides bipunctatus, Barbus capito, Rutilus frisii kutum and Vimba vimba persa were observed in total stations. Among identified fish species in the study, were 9 species belong to migratory (anadromous), 19 species belong to freshwater resident and 2 species had both forms. 7 species were identified as alien or exotic which some of them had high dispersion and high abundance and therefore they are dangerous for native fish species and migratory fishes restoration.

Microorganisms such as Salmonella, Listeria, Campylobacter and etc. create Food born diseases in human, which cannot be prevented by controlling and food safety systems. In this study, 30 samples are taken from slaughtering stages and prevalence rate of positive coagolase Staphylococcus aureus, Salmonella and Clostridium perferings and contamination level of Staphylococcus aureus have been studied using standard methods. T- TEST for quantitatives data and Mcnemar test for qualitatives data has been used. Increasing the mean of Staphylococcus aureus contamination level after each of premises such as defethering , eviscerating and immersion chilling are significant (p<0.05). However, decrease of Staphylococal contamination level in cold water spray washing is not significant (p>0/05). Staphylococal contamination frequency after immersion chilling in camparison with cold water spray washing and Salmonella contamination frequency after eviscereating in comparison with defethering show significant increasing (p<0.05). Clostridium perferingens contamination has been observed in samples. But variation of its prevalence rate and paired test of it, s stages are not significant (p>0/05). Finally, results show that the stages of defethering, eviscerating, cold spray washing and immersion chilling are seriously critical points in sloughter house.

This study was conducted to determine the effect of wet diets in compensatory growth period on live weight, feed conversion ratio, feed intake and carcass characteristics of broiler chickens in Ramin Agriculture Research Station. 240 chicks from Arian strain, were randomly selected and divided into six experimental groups. Treatments were assigned in a Completely Random Design with Factoria l(3*2) arrangement, including 3 levels of restriction (0%, 10% & 20%), and 2 forms of ration (dry and wet), with 4 replicates, and 10 chicks per each replicate in treatment. Results showed that final live weight gain in 7Th week for 0%, 10% and 20% of restricted treatment, were 2031/13, 1905/13 and 1907 grams respectively. The aforementioned reducing levels decreased weight gain significantly (p<0.05), while change of ration from dry to wet, had no effects on final live weight gain (p<0.05). Feed intake of 20% restricted treatment was significantly (p<0.05) lower than other treatments, as well as the feed intake of wet ration that was less than dry ration. Feed conversion coefficient of 0% restricted treatment was less than 10% and 20% restricted treatments, but not significantly (p<0.05). Feed conversion coefficient of wet ration was less than that of dry ration (p<0.05). There was no significant difference between treatments (p<0.05) in terms of carcass weight, breast weight, thigh weight, intestine weight and abdominal fat weight, .Intestine weight was significantly different in wet ration with other treatments (p<0.05).

This experiment was designed to investigate the effects of oil on broilers performance during grower and finisher periods. To carry out the experiment 14 days old male chicks (Ross strain) with average weight of 195±10 were selected and based on completely randomized design were divided between cages randomly with 4 replication. The chicks received different fat levels (0, 2, 4, 6 %). At the end of the experiment one chicken from each replication was selected randomly and killed to investigate the carcass analysis. There were significant difference in final body weight and weight gain between the treatments (p<0.05). So that there were increasing trend with adding fat. There were non significant difference in feed consumption between the treatments, but there were increasing trend with adding fat. There were significant difference in feed conversion between the treatments, Then adding fat improved this trait (p<0.05). There were non significant difference in abdominal fat pad between the treatments, but there were increasing trend with adding fat. Dietry fat inclousion led to increasing body weight, feed intake and optimized feed conversion ratio.

The comparison of diversity and abundance of macrozoobentos at three chinese cyprinids fish farms sampling for determination of abundance and diversity of macrozoobentos, at tree 1- hectar farms of chinese carps in Gulian province was done every 15 days for 8 months. Used a 272 – Cm2 grab, for sampling of sediments at entrance, middle part, and exit of farms. Resalts showed 3 groups of macrozoobentos in sediments of farms. That including: Tubificidae, Chironomidae and Gastropoda. The total abundance percent of this groups was 77.18, 20.26, and 2.57 respectively. The mean abundance of Tubificidae in 3 farms in Spring, Summer and Autumn was 279, 864 and 278 ind/m2 respectively. Frequency of this group in Summer was significantly more than spring and Autumn (p<0.05). The mean abundance of Chironomidae in spring, summer and Autumn in 3 farms, was 221, 87, and 64 pieces/m2 respectively. The abundance of this group in spring was significantly more than Summer and Autumn (p<0.05). The mean biomass of chironomidae in sediments of farms in Spring, Summer and Autumn was 2.3, 0.67 and 0.4 g/m2 respectively. The biomass of chironomidae in spring was, significantly more than Summer and Autumn (p<0.05). The mean abundance of Gastropoda in sediment of 3 farms in Spring, Summer and Autumn was 4, 14 and 27 ind/m2 respectively. The abundance of this group in Autumn was significantly more than Spring and Summer and in Summer more than Spring. On survey of intestine content of common carp (Cyprinus carpio), seen Chironomidae and gastropoda. The percent of total organic matter at entrasnce to exit of farms increased. The total percent of T.O.M in entrance, middle and exit of farms was 8.67, 10.97, and 13.21 respectively. In determination of grain size and composition of sediments of farms, the percent of sand plus silt and clay was 61.31 and 36.34 respectively. Also the mean of phisical-chemical parameters, such as, water temperature, dissolve oxygen, transparency, and pH were, 26.25 oc, 7.64 mg/l, 34.22 cm and 7.90 respectively.

Leptospirosis is a world-wide zoonotic infection occurring in animals and human. Rodents and wildlife are a major source of infection. Clinical signs in cattle include fever, heamoglubinuria, jaundice, abortion, mastitis, reduced in milk production, reproductive failure and death. In this survey, the seroprevalence of Leptospiral infection in dairy herds and the relationship between seroprevalence and animal and environmental factors in Tabriz were investigated. 229 serum samples randomly collected from herds since December 2002 to November 2003. Sera were tested by MAT using live Leptospira interrogans serovars: Hardjo, pomona, icterohaemorrhagiae, grippotyphosa, canicola and ballum. Twenty four percent of sera were seropositive against one or more of studied serovars. Pomona and grippotyphosa were detected as the most-prevalent serovars with 34.2% and 32.9% respectively. All of sera were seronegative for hardjo. The Chi-square method used for seasonal tend of seroconvertion against this serovars. The risk of seroprevalence against leptospira was significantly higher in winter and autumn. In farms, where rodents had been observed, and or moisture were high, the seroprevalence was higher than others. Also most seropositive cases were observed in 3-4 years old cows. According to presence of serological infection in dairy herds, use of prevention factors were suggested.

In order to illustrate the origin of occurrence of congenital malformation in rainbow trout, in the present study, the percent and type of malformation in brother-sister mating were compared with those of control. For this propose two pair of breeders from different stocks have crossed to produce sister-brother line. For estimation of mean deviation between family and non family mating of traits, analysis of variance have used and hatchability rate was lower in family mating but was not significantly different (p<0.987). In this study the type and percent of abnormal larvae, fingerling and marketable size fish were investigated. Pug head, curved body, shortened tail and enlargement of the yolk sac was the character of malformations at larval stage. The difference of mean at the end of yolk absorption and abnormality at stages of 150, 225 and 300 days have not shown any significant difference (p<0.960, p<0.774, p<0.775, p<0.730) respectively. The fingerling of 450 mg, one month after active feeding stages, had only 5% abnormal consistent of shortened body length (92%), pug head (2%), shortened operculum (5%) and lump back(1%). It was shown that even in crossing between close family (sister-brother), the abnormality was not significantly higher than normal mating, therefore it may be due to environmental factors of management problems.

Tagh (Haloxylon sp.) is one of the plants in desert that prevent soil erosion and also moving of sand. It also provides fuel for people and feed for livestocks. The young branches (browses) of Tagh is a good feed for goat and camel. The total Tagh planted area is estimated to be 1.9 Million hectar in Iran. In addition, pruning of Tagh from 10-15 cm from top, stimulates renewal of plant and the cut leaves is being consumed by herbivorous animal. In this experiment samples were collected randomly from three intensive cultivated planted areas in Kerman province. The samples were choped to 1-2 cm pieces. Samples were mixed to get homogenous and then were used for chemical analysis. The biological assays includes: Dry Matter Digestibility using in vivo and in vitro and nylon bag degradability (in situ) methods. The mean and standard deviation for chemical compositions and biological assays. Wereas follows DryMatter,93.37±0.73; Organic Matter,78.93±2.22;CP,8.18±0.76;EE,0.71±0.15;Ash, 14.44 ± 1.91; CF,28.22 ± 5.53; ADF,32.93 ± 1.04; NDF,49.35 ± 2;NFE,37.10 ± 2.67; LIG10.9 ± 0.11;Tanine,0.14 ± 0.02;Total Phenolic Compounds, 0.7±0.04 and ME, 1091.23±140 Kcal/kg DM. Mean of dry matler digestilility using in vivo method was 40.55±2.89 and in vitro digestibility for dry matter, organic matter and Organic matter in dry matter were 41.93±3.25, 34.36±4.44 and 29.50±3.81 percent respectively.

The limnological study of Zohreh river was initiated along PRIM bridage, at the opening section of Kohgiloyeh - Boyerahmad province and the district was continued to the exit ramp of province close to Hydar - e - karar village. This part of the river is 100 km long. Sampling was started from the Fall of 2003 and continued to the Winter of 2004. Sampling was done from three specific stations. Samples were provided from given stations monthly to determine water, plankton and benthos factors. They were transferred to lab to be analyzed. At the same time, some physical and chemical factors in terms of O2, pH and temperature were recorded. The result indicated that the amount of calcium and electrical conduct, phosphate was increasing. Also, 24 phytoplankton groups, 2 zooplankton and 11 benthos were recognized. One important finding is that there is no significant statistical difference in terms of frequency and number of plankton benthos, among three sections.Besides proper and acceptable physical and chemical factors for reproduction of carps were the same.

With attention to studying about pollution environment and the andemic especies in the zone, sampling of Pinctada radiate, water and sediment from 4 stations in Hendorabi island have been conducted in 2002 Summer after biometrics, evolution of Pinctada radiate, the chemical analysis of the samples Pb and Cd were done by using intermined with the help of Atomic Absorption Spectropbctometer (A.A.S). Result of the analysis Stated that: The mean value Pb and Cd in sediment was inorder 4.62 and 1.11 (ppm) and in water 3.67 and 0.837 (ppm), which comparing the estandard global suitable for sea animals and aquaculture , and the mean value Pb and Cd in Pinctada radiate was inorder 10.87 and 2.08 (ppm) respectively in shell, which comparing to the EPA standards, The mean value Pb not suitable, and Cd suitable for sea animals. There was not significant different between weight and size of Pinctada radiate with their absorption and accumulation of heavy metals. But there was a significant different and positive correlation between Pb and Cd content in water.

D. salina is a unicellular green alga. The individual effects of Cd2+ (0.5 mg.L-1) at different concentrations of NaCl (1, 1.5 and 2M) on the growth (cell number and total chlorophyll content) of Iranian strain of Dunaliella salina were examined during 36 days of treatment. The results show that increase in salt concentration caused significant increase in cell number and chlorophyll content of D. salina. It seems such increase in growth rates is due to formation of CdCl 2 and decrease of uptake of Cd2+ by cells.

Monthly sampling of five stations in Dez dam lake was carried out for fish analysis from Jan. 2002 to Dec.2002 in Dez river (after dam ). In this study 12 fish species , belong to 3 family , were identified and cyprinid fishes with nine species were the most dominant family. The most abundant fishes was observed in Summer. Capoeta trutta, Barbus grypus and Barbus esocinus were the most species respectively. Most of fishes were omnivor and also planktivor, carnivor and detrivor fishes were observed. Spawning of the most species is occurred the late Winter and Spring and some species was spawning in different seasonal time. Indices: diversity, richness , evenness and dominance 1/11 , 1/05 , 0.45 and 0.46 were determined respectively.

One of the major draw backs in the live food industry is the high cost of culture medium. In the present research project effect of three types of culture medium in three different ranges of light were studied on growth of Tetraselmis suecica. The experimental set up of the project included nine treatments in triplicate of three different culture medium which were studied in three different ranges of light 500, 1500, 2500 lux. The result obtained showed a significant variation between different treatments of light and that growth has a direct relation with ligth intensity. On the other hand the significant difference was recorded for the culture medium treatments and the results indicates that vitamins have a significant role in the quality of the culture medium. Howevere no significant difference was observed between the usage of Guillard medium vitamins and group B- vitamins.

The effects of enriched rotifers with highly unsaturated fatty acids and vitamin C on the growth factors and survivals of fenneropenaeus indicus has been evaluated during larval feed stage. Larvae were fed newly hatched Artemia nauplii (Control); rotifers cultured on Chlorella sp. algae (treatment 1); rotifers enriched with emulsion cod liver oil (treatment 2), and enriched rotifers with emulsion cod liver oil and ascorbyl palmitate (treatment 3). Most concentrations which at a level of (0.05) with treatments 1 (0.4) and control have shown a significant difference. Teh ratio of DHA/EPA among dietary treatments had a significant difference and the most difference occurred in treatment 2 (0.65) and 3 (0.72) and and the least ratio has been recorded for treatment 1 (0/05) and control (0/01). The level of EPA and the ratio of n-3/n-6 among the treatments shows exactly the reciprocal with the values mentioned above so as the most values have been observed in treatments 1 and control while treatment 2 and 3 had the least values. The variation of these fatty acids in the tissues of larvae fed withy dietary treatments is in the same order of the above. Therefore on this basis most of the growth and the survival enhancement observed at postlarval 1 (PII) in treatments 2 and 3 have been enriched with unsaturated fatty acids and unsaturated fatty acids with vitamin C respectively. This shows a significant difference with treatment 1 and control (P<0.05). Also the lowest growth and survival rates have been experienced in control treatment. But in post larval 5 (PL5) the most growth and survival rates with respect to treatment 1 in which their difference has also been significant (p<0.05).

Phenytoin sodium is one of the effective drugs for healing of skin lesions in animal and human. However, the pathologic effects and dosage in fish has not been investigated yet. In this study the pathologic and clinical effects of phenytoin on gill, liver and kidney of gold fish were investigated. 140 fish were disinfected with 3% sodium chloride solution and then devided into 4 groups (35 fish in each group) including, control, group 1, group 2 and group 3. Phenytion sodium was administered for 20 days at different dosage: 5, 10 and 15 mgr/lit in groups 1, 2 and 3 respectively. Tissue samples prepared from liver, kidney and gill of all group and stained with H&E staining method. Histopathological lesions were found in groups3. There were congestion, hemorrhage, necrosis of hepatocytes and fibrinous exudate in liver. There were also congestion, hemorrhage, necrosis of renal tubules and increased melanomacrophage in kidney and congestion of gill, epithelial and chloride cell hyperplasia, hemorrhage and fusion of lamella were observed. Clinical sings were recorded for all groups during the study. Groups control and 1 were normal, but fish of group 2 and 3 showed clinical sings such as ataxia, depression, anorexia, constipation and marked increase of mucous secretion in the skin and gill.

The propagation of Rutilus frisii kutum started in 8 March 2004. This trend occurred in four rivers such as Shirod, Tonekabon, Tajan and Goharbaran. The initial temperature was 7-8°C and the end temperature was 25-26°C and this trend ended for two months. The propagation ended in 19 March 2005. During this time, 7168 and 18218 number of female and male brood stock specimens of 25386 brood stocks utilized for propagation. The mean of length, weight and condition factor of brood stock fish female and male were (43.75±1.5, 36.5±1.91) cm, (1189.5±40.36, 678.13±115.94) g and (1.42±0.11, 1.38±0.05) respectively. The total of Rutilus frisii kutum eggs during 62 days was 4931.01 kg. The highest eggs obtained (3189.31 kg and 64.7%) of Shirod River such as the highest eggs were obtained in end of March and initial April (2369. 55 kg, 48% of total eggs). The percentage of survival of eggs in four rivers was measured 94.5, 95.1, 87.7 and 96.9 respectively. The comparison between ovary weight and gonadal somatic index (GSI female), absolute fecundity, relative fecundity and hatching rate in four rivers were studied. There was only difference in hatching rate below significant rivers (Shirod River: a, Tonekabon River: b, Tajan and Goharbaran River: c) (p<0.01). The total lengths and weight in larval (Newly hatch) were TL=6±2 mm and W=2±0.2 mg respectively. Condition factor is measured to cf = 0.92 ± 0.15 the length and weight of three days larva were measured TL= 7 ± 2 mm and W= 2.7 ± 0.1 mg respectively. The condition factor calculated cf = 0.78 ± 0.1. The fry surveyed from the parasite observation before releasing into the river. The infection severity was type one in all of ponds in this survey, 4366 number of fish sampled from 66 ponds and 500 numbers of fish (11.45%) were infected to type of parasites. These parasites included in Diplostomum, Dactylogyrus, Butriocephalus, type’s nematode, Trichodina and Epistelis. The 16942454 numbers of fish produced in below standard weight (Lower one grams) because the reason of incorrect management in fish culture. These numbers were equal to 19.9% of total released in Mazandaran province in 2004. . The highest frequency of weight related to weight class of 1-1.5g. This value was equal to 68.1% totally, 81% ( 62905247 numbers) of total fry released has suitable weight for releasing.

Pathogenesis and efficiency of LD50 concentration inclusion of nuclear polyhedrosis virus orally was evaluated in 36 Iranian silkworm varieties. After extracting hemolymph of infected larvae to grasserie disease, polyhedrosis virus was purified and fed to larvae infected consumed leaf orally at the beginning of fourth instar. Treatments of this investigation were conducted in generalized linear models with five replications (each replication including 250 fourth instar larvae). The treatments included inoculation or without inoculation of LD50 concentrations of polyhedrosis virus orally, and varieties included 36 silkworm varieties (21 pure lines and 15 hybrids). Furthermore, the studied traits included survival larvae number, survival pupae number and pupation rate. From obtained results, the Chinese origin lines had higher resistance than Japanese origin lines. Furthermore, the resistance traits were highly affected by heterosis, so heterosis percentage was higher in infected environment. In control environment, heterosis for survival larvae number, survival pupae number and pupation rate was 12.09, 16.94 and 3.6 percent, respectively and in infected larvae was 11.67, 27.13 and 14.26 percent, respectively. The averages of these characters were reduced 33.44 larvae, 80.35 pupae and 32.42 percent, respectively as a result of infecting the varieties with NPV (P<0.0001). Thus, the efficiency of LD50 concentration inoculation of nuclear polyhedrosis virus orally is very high for infecting larvae.

The objective of this research was to study the digestive potential and kinetic of digesta in Iranian camel and sheep and compare those factors between the two animals. Four fistulated intact camel and 4 fistulated intact sheep were used in this research. A replicated 2×2 Latin square design was used to assess the effect of two different rations including 0% and 30% concentrate on digestive potential and kinetic of digesta. When two different rations including 0 and 30% concentrate was offered to camel a significant difference was observed (p<0.05) in pH values and VFA concentrations in the rumen but such differences was not detected (p>0.05) in sheep. The difference in VFA concentrations between sheep and camel was significant (p<0.05) but no such difference (p>0.05) was observed in ammonia nitrogen concentration and pH values in rumen between the two animals. In camel, the digestibility of NDF and ADF of the ration containing 0% concentrate was higher (p<0.05) as compared to the ration containing 30% concentrate. In sheep and camel the pH value and urea concentrations of blood were not affected by the two rations but the difference between the two animals was significant (p<0.05). The results showed that in camel VFA production in the rumen and potential of ammonia nitrogen recycle was higher as compared to sheep.

Rapid detection of Leptospira is one of necessities in treatment of this zeonostic disease. The unability of serologic methods for detection of these bacteria in initial steps of infection (these methods needs to progress of infection and development of antibody) has candidated molecular methods based on PCR, as an important goal for rapid detection of Leptospirosis. Because of the exiestance of this disease in Iran, it seems a necessity to program for control and treatment of it. For this purpose, the first step is the detection of these bacteria. In this research we developed two PCR methods for detection of the bacteria. Three identified dominant serovars of Leptospira in Iran L.canicola, L. sejroe hardjo, L. grippotyphosa were used in development of PCR. The first one was targeted to a non coding locus producting 285bp with G1/G2 set of primers and the second was part of a novel repetitive sequence producing a 571 bp product with primers 590DIR1/590REV2.Comfirmation of PCR product was done with RFLP. AluI and MboI were used for 571 and 285 bp products, respectively.

An experiment was conducted to evaluate the effects of chemical treatment of barley grain with sodium hydroxide (NaOH), ammonia and formaldehyde on blood metabolites and productive performance of lactating Holstein dairy cow. In this experiment a balanced change-over design with 16 cows (in mid lactation stage), four rations and four periods (21 day per period with 7 days interval) Were employed. The experimental diets 1 through 4 contained untreated barley, barley treated with NaOH (3.5 % weight), Formaldehyde (0.4 % weight) and Urea (3.5 % weight) respectively. The cows were fed individually ad libitum twice per day. Daily milk yield was recorded and samples of the milk were taken twice per week, also samples of rumen liquors, urea and blood were taken in end of each periods. In this experiment dry matter intake, body weight change, and fat corrected milk of cows were not affected by experimental diets. Daily milk yield differences were statistically significant between rations (p<0.01). Fat and total solid percent of milk were decreased by feeding rations 2 and 4 (p<0.05). The concentration of lactose, protein and solid non fat in milk were not affected by rations . The pH of cows rumen liquor and urea were significantly different between rations. Also concentration of glucose, calcium, phosphorus, sodium, cholesterol, triglycerids, magnesium, potassium and total protein in blood plasma of cows received different diets were not different (p>0.05) but blood urea nitrogen concentrations of cows received diet 4 were higher than other groups. On the basis of these data, we can conclude that feeding barley treated by NaOH or Urea increase milk yield and decrease milk fat percent but barley treated by formaldehyde only increase milk yield.