Journal of Water and Soil Science
Year:2008 | Volume:12 | Issue:43 (SPECIAL ISSUE IN SCIENCES AND TECHNOLOGY OF FOOD AND ANIMAL)|Papers Count:25

Baking is obviously one of the most important stages of bread production. Baking time and oven temperature have greatest effects on bread quality. The aim of this study was to investigate the effect of baking time and temperature on bread quality and its shelf life. In this study, the effect of three rates of oven temperature (top and under bread surface) and baking time levels on bread quality and its shelf life was investigated. Baking temperature and time were 300 and 280oC for 3 minutes and 20 seconds, 300 and 350oC for 2 minutes and 30 seconds and 350 and 380oC for 2 minutes respectively. Soluble starch and staling factors of the samples were measured. The data was statistically analyzed by complete randomized design and comparison was made between the means via Duncan’s multiple range test at 5% level. The results showed that the bread baked in various time–temperature conditions were different in moisture content. The bread baked at lower temperature and longer time had the lowest moisture. Also, the result showed that the amount of soluble starch increased by increasing the baking time and decreasing the baking temperature. The bread baked in various baking conditions showed significant differences in staling rates. Lower baking time and higher baking temperature caused the lowest bread staling rates.

In this research the effects of two common cooking processes of foods, namely, autoclave and extrusion cooking on the molecular and physicochemical properties of wheat starch were investigated. The results showed that the solubility of the autoclaved samples increased with increasing the temperature while their relative viscosity decreased. These findings indicated that the molecular degradation of starch could occur during autoclave cooking. Moreover, constant sedimentation coefficient (s-value) of amylose and decreasing the s-value of amylopectin with increasing the temperature further confirmed degradation of amylopectin in particular. The results of the extruded samples showed that reduction in water content may increase the shear and pressure produced during extrusion cooking, which in turn could cause several changes in starch properties including degradation of starch granules, formation of amylose-lipid complex and degradation of amylopectin (as determined by reduction of the s-values). Also, reduction in water content could increase the puffiness of the final products and reduce the cold-water and final viscosities of the extruded samples. These observations may indicate the importance of the molecular studies of starch for better interpretation of physical changes of the final products.

Based on the official statistics of industries and mines organization about 1000 tons of waste of spaghetti in spaghettiproducing plants, and around 7500 tons of waste in chickpea pre-cleaning plants are produced annually in the East Azarbayjan province. In order to determine chemical composition and metabolizable energy of the above mentioned waste, at first classified random sampling from 10% of plants was performed. Then amount of dry matter, crude protein, crude fiber, ether extract, NDF, ADF, and gross energy were measured according to AOAC (1990) procedures. The amounts of crude protein, crude fiber, ether extract, NDF and ADF, for spagettti waste were, 12.7, 0.2, 2.4 , 1.7 and 0.1 percent, respectively, and for chickpea pre-cleaning were 30.2 , 17.8 , 8.7 , 32.3 and 22.4 percent, respectively. The waste of spaghetti was mixed with basal diet with the ratios of 0, 15, 30, 45, 60, 75, 90, and 100%. The waste of precleaning chickpea was mixed with basal diet with the ratios of 15% and 45%. Then, in order to determine different kinds of metabolizable energy (AME, AMEn, TME, TMEn) of waste, 30 grams of mixed feed were force-fed to 4 adult Leghorn-type roosters, according to the method described by Sibbald. The amount of AMEn of spaghetti waste in different levels was 2915, 3535, 3937, 3643, 3728, 3671, 3687 and 3717 Kcal/kg, respectively and the amount of AMEn of metabolizable energy of checkpea pre-cleaning in two levels was 2677 and 2279 Kcal/kg, respectively. Statistical analysis showed that there were significant differences between AMEn of wastes. There were significant differences between amount and kinds of metabolizable energy in different levels. The highest amount of AME was observed to be in 30% ratio of spaghetti waste relation to basal diet. There were significant differences between amounts of metabolizable energy of checkpea pre-cleaning in two levels. The highest amount of AME was observed to be in 15% ratio of checkpea pre-cleaning waste related to basal diet.

Chitosan, a deacetylated derivative of chitin, is found in crustacean, arthropod, shell fish and shrimp shell as well as cell walls of some fungi. The objective of this research was to investigate the antimicrobial property of chitosan and possibility of its use as a natural preservative in mayonnaise. In this study, chitosan was produced by chemical method from shrimp shell. The antimicrobial activities of chitosan against Salmonella enteritidis and Lactobacillus plantarum were explored by calculation of the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) in media supplemented with 0.1, 0.2, 0.3, ..., 0.9, 1 mg/ml chitosan adjusted to pH 5 or 6 . The MIC and MBC of chitosan differed with pH and bacterial type, ranging from 0.4 to 1 mg/ml. Antibacterial activity of chitosan was inversely affected by pH, with higher activity at lower pH value. The sauce containing 0.1, 0.2 or 0.3% chitosan was inoculated with106 CFU/gr S. enteritidis or L. plantarum and stored at 5oC and 25oC for 8 days. The inhibitory effect of chitosan concentration was increased from 0.1 to 0.3%. This investigation demonstrated that 0.2% chitosan can be used in mayonnaise as a new preservative.

The aim of this study was to use Alpha-amylase termamyl 2-x for maltodextrin production from corn starch and evaluate its industrial uses. Based on the results obtained in laboratory, this process was accomplished in pilot plant scale. The process included preparation of starch suspension, pH adjustment, addition of enzyme, heating under stirring, continuous control of DE and Brix, enzyme inactivation in adequate DE, separation of soluble sections by centrifuge and finally spray-drying of the maltodextrin liquid. In this investigation, the amount of DE at dry matter was calculated under three enzyme concentrations (0.2, 0.25 and 0.3 ml of Alpha-amylase termamyl 2-x per Kg of starch) and in three different hydrolysis temperatures (60, 65 and 70oC) at constant pH (6). A completely randomized design with factorial arrangement and 5 replications was conducted. To study the relationship between DE and different parameters, multiple linear regressions was used. Finally, for approximation of DE (based on enzyme concentrations, temperatures and hydrolysis times) a regression model was used. The results from different enzyme dosages at the same temperature and time of hydrolysis differed significantly (p£ 0.05). Meanwhile, the best enzyme concentration and hydrolysis temperature for maltodextrin (high DE) production after 300 minutes were 0.25 ml of enzyme per kg of starch and 70oC, respectively. In these conditions, the least residual starch concentration and residual Alpha-amylase activity were observed.

Effect of semihydrogenated vegetable oil (shortening) and sodium stearoyl lactylate (SSL) on retarding Barbari bread staling was investigated in this study. Three levels of 2, 3 and 4 percent shortening and SSL in two levels of 0.5 and 1 percent of flour were used in this research. Treatments included control sample (without shortening and SSL), bread with only shortening, bread with only SSL, and bread with 0.5 percent SSL and 3 percent shortening. Organoleptic properties and staling factors of the samples were determined. The data was statistically analyzed by complete randomized design and means comparison was done by Duncan’s multiple range tests (5% level). The results showed that the breads containing SSL and shortening were significantly different in organoleptic properties and samples with 0.5 SSL and 3 percent shortening had the highest quality. The results of staling test showed that samples with 0.5 percent SSL and 3% shortening had the lowest staling rates.

This experiment was carried out to determine the nutritional value and the effect of different levels of tomato seed and pomace on performance of broilers. Corn grains and soyabean meals were replaced by tomato seed at levels of 6, 12 and 18 % and tomato pomace at levels of 3, 9 and 12 %. At first, the metabolizable energy of tomato seed and pomace was determined by the Sibbald method. In this experiment, 504 commercial broiler chicks (Ross 308) were used for 8 weeks (from 7 to 56 days old ) in a completely randomized design in 7 treatments with 4 replications per treatment. After 21 days, protein digestibility of the ration in illeum was determined. Apparent metabolizable energy was 3394 kcal/kg for seed and 2329 kcal/kg for pomace and crude protein was 31, 22.7, respectively . The result showed that body weight, protein digestibility of rations in illeum and carcass characteristics were not significantly different. Best energy and protein conversion efficiencies were obtained at the level of 6 % seed and 9 % pomace that were not significantly different from control treatment. Cheeks fed with seed and 12% tomato pomace had significantly (p<0.5) higher feed intake than control group.The difference between diets containing 6% seed and 3% pomace and control group was not significant regarding feed conversion ratio.

This experiment was conducted to evaluate the effects of 7 experimental diets (2 diets containing 100 and 200 mg vitamin E (VE)/kg and 5 diets containing 0, 0.5, 1, 1.5 and 2% green tea powder (GTP) ) on performance and breast and thigh meat oxidative stability of broiler chicks during different storage times. A total of 364 male broiler chicks were divided to 28 groups of 13 chicks each. Then every 4 groups were randomly allocated to one of experimental diets. During the experimental period (7-42 days of age) feed intake, weight gain and feed conversion of the experimental groups were measured. On day 42, 2 birds from each replicate (8 chicks per treatment) were weighed, slaughtered and their muscles of breast and thigh were minced and stored at 4ºC. Meat oxidative stability of the samples was determined on days 0, 6 and 9 of storage time using thiobarbituric acid test (TBA test). The results showed that addition of the VE and GTP to diets tended to significantly (P<0.05) reduce live body weight, weight gain, feed intake and abdominal fat accumulation and increase feed conversion ratio and pancreas size compared to control group. Increasing storage time from 0 to 6 and 9 d significantly (P< 0.05) increased TBA number. Lipid peroxidation and TBA values in thigh muscle were significantly (P<0.05) higher than breast muscle (1.46 vs. 0.359 mg Malonaldehyde/kg meat). Different dietary levels of GTP in diets did not have any significant effect on prevention of meat lipid peroxidation. In addition, the values of TBA in meat of the groups fed diets containing high levels of GTP significantly (P<0.05) increased in comparison to control group and those fed VE supplemented diets.

To study the effect of age on camel hair characteristics and their phenotypic correlations in Semnan province, 95 dromedary female camels (aged 1-20) were selected. Hair samples were taken from right flank in mid spring. Least square means were analyzed at different ages, and also 4 age groups by one-way and general liner model (GLM) using SAS software package. Analysis of variance indicated a significant effect of age group on staple length, fiber diameter, coefficient of variability of fiber diameter, interrupted and continuous medulla and non-medullated fibers in inner coat and yield percentage (P£0/05). The young camels had more inner coat and non-medullated fibers than other ages. The diameter least square means of 1-2 age group (18.54±1.47 um) was significantly different (P<0.005) from 3-6, 7-10 and >11 age groups (22.59±0.67, 23.19±0.57 and 24.5±0.61 um, respectively). There was a significant difference (P<0.05) in shoulder, flank and total staple lengths between 1-2 and other camel age groups (P<0.0001). The results indicated that fractionation of camel hair according to age grouping (young camel separated from adult) can improve supply to market and manufacture processing.

A total of 1256 records associated with body weight and Cashmere at different ages (birth and 3 and 9 months) obtained from 754 Cashmere goats were used to estimate the genetic parameters in southern Khorasan province during 2000-2003. A set of univariate animal models including additive and maternal genetic effects and maternal permanent environmental effects as well as the fixed effects of year and month of birth, sex, birth type and dam age (linear and quadratic covariates) and kid age (linear and quadratic covariates) was fitted. Co/variance components were estimated by restricted maximum likelihood procedure using Powel algorithm in DFREML software. For the body weight at 0, 3 and 9 months, models two, one and three were recognized as the appropriate models. For these models, direct heritability estimates were found to be 0.09, 0.11 and 0.09, respectively. For birth weight and weight at month 9, the magnitude of c2 and h2m were 0.18 and 0.00, respectively. For average daily gains during 0-3 and 3-9 months of age, direct heritability based on the model one was 0.16 and 0.05, respectively. Direct heritability of Cashmere was found to be 0.02. Applying repeatability model, the estimates of heritability and repeatability of Cashmere were 0.16 and 0.53, respectively. Genetic trends for birth weight (0.0175kg/year) and weight at month 9 (0.02065kg/year) were positive and non-significant. A negative non-significant statistical genetic trend (-0.00537kg/year) was found for Cashmere during the period of time.

24 Holstein cows and 16 Holstein heifers in close-up period were used to evaluate the effects of ground wheat grain in prepartum diets on health and performance of dairy cows. Cows were blocked based on parity (three groups) and then randomly assigned to two experimental diets. Study rations included a treatment containing wheat with 1.62Mcal/kg NEL, 14.8%CP, 42.1%NFC, and –64meq/Kg DCAD and another treatment was composed of barley and wheat bran with 1.59Mcal/kg NEL, 14.8%CP, 38.2%NFC, and –48meq/Kg DCAD. Cows were fed experimental diets in group, on average 24%4 days prior to parturition, and they were fed the same ration up to day 21 postpartum. Average feed intake prior to confining, milk yield and composition, blood metabolites, feeding and chewing activity, urine and feces pH, placenta weight and time of its omitted, pregnancy duration, parturition status, body weight and BCS, and metabolic disorders were evaluated in this research. Average feed intake for wheat treatment was more than barley and wheat bran treatment (11.56 in contrast to 10.74, Kg DM), but it was not statistically significant. Milk production in wheat treatment had no significant increase. Milk fat yield in wheat treatment had a higher value compared to barley and wheat bran treatment (P<0.025). Blood calcium in wheat treatment was significantly higher (P<0.005), and blood glucose in wheat treatment was significantly higher than barley and wheat bran treatment (P<0.011). Changes in body weight and BCS, pregnancy duration and parturition status were not significantly different between treatments. Urine pH in wheat treatment had a significant decrease in the last week prior to parturition (P< 0.003).

This experiment was conducted to compare the effect of probiotic thepax in diets with different quantities of protein on the performance of Cobb-500 broiler chickens and their serum antibody level against Newcastle disease vaccine (NDV). Two basal diets were formulated based on NRC (1994) recommendations which had NRC (sufficient protein diet) and 90% NRC (low protein diet) for protein. The basal diets were then supplemented with 0 and 0.1% probiotic, resulting in a 2´2 factorial arrangement with 2 levels of probiotic (0 and 0.1%) and 2 levels of protein (NRC and 90% NRC). Each of the 4 dietary treatments was fed to six replicate groups of 20 chicks. The results of experiment showed broilers fed low protein diet without supplementation of probiotic had lower performance than other treatments. Supplementing low protein diet with 0.1% probiotic significantly improved body weight gain and feed conversion ratio, as did those which were fed diets with sufficient quantities of protein. Reducing dietary protein level increased abdominal fat content, but had no significant effect on other carcass composition. Supplementing of probiotic significantly reduced abdominal fat percentage (P<0.05). The utilization of probiotic in low protein diet resulted in increasing serum antibody level against NDV, but sufficient protein diet produced no such effect. Also, the results of experiment indicated that probiotic added to low protein diet improved broilers performance and immune response. However, these effects were not observed in adding probiotic to diet with sufficient quantity of protein.

This experiment was conducted to determinate the effects of different levels of Amet protein binder (0, 1.5, 3, 4.5 and 6) in substitution for fish meal on performance and digestibility of nutrients. This study used 400 broiler chickens (Ross strain) with 5 treatments of 4 replications in a completely randomized design. Weight gain, feed consumption, and feed conversion ratio were determined at the end of each phase of the experiment. In addition, the digestibility of nutrients and apparent metabolizable energy (AMEn) of the whole diets were measured by sampling excreta. The results showed that levels of 4.5 and 6% of Amet significantly decreased feed consumption (P< 0.01). Feed consumption was higher for 1.5, 0 (control), and 3% Amet in diets, respectively. At the starting phase, 1.5% Amet treatment improved feed conversion ratio (P<0.01). The treatments 6% and 4.5% Amet increased feed conversion ratio in all phases of experiment and the final phase. Percentages of abdominal fat were not significantly affected by treatments. Replacement of different levels of Amet except the treatment 6% had no significant effect on percentage of carcass. The level of 1.5% Amet versus control significantly increased AMEn (P<0.01). It seems that the inclusion of 1.5% Amet binder in the diet can positively affect performance and AMEn of diet in broiler chickens.

This experiment was conducted in a completely randomized design with five treatments and four replications. There were 15 chicks in each replication during the first 30 days and 10 chicks from day 30 to the end of experiment. Diets were control (without probiotic and dry whey) and levels 500 and 750 gm/ton probiotic, and each level contained two diets with and without %2 dry whey. Daily weight gain, feed intake, feed conversion rate at the end of each experiment and carcass characteristics including weights of neck, Liver, wing, heart, and abdominal fat on the 49th day of experiment were measured. Chicks which received diets with 500 and 750 mg/ton probiotic +%2 dry whey were significantly heavier than other chicks (p<0/05) between 0-21 days. Feed intake significantly increased during 3 periods including 0-21, 21-42, and 42-49 days of experiment, when chicks used diets with dry whey, especially diets containing 750 gm/ton probiotic (P<0/05). Blood plasma cholesterol significantly decreased when chicks used diets with dry whey. Also an increase in white cells was observed in days 21 and 35. Treatments did not have any significant effect on feed conversion rate, mortality and carcass characteristics (P>0/05).

This experiment was conducted to determine the phosphorus relative bioavailability of eight samples of dicalcium phosphate. Bioavailability of Samples of dicalcium phosphate was evaluated and their effects on performance of laying hens were investigated. Commercial samples of Dicalcium Phosphate were provided from the following manufacturers: Iran Phosphate, Partove Bashash, Godaze, Poya Khojaste, Dan Razy Kimia, Phosphore Iran, Golbar Shimi and Dan Avar. The Parameters measured were shell strength, shell thickness, shell ash percentage, shell percentage, shell calcium and phosphorus percentage, Haugh unit, egg weight, egg production, egg output, feed consumption, feed conversion ratio, tibial ash, and tibial calcium and phosphorus. One hundred and six, and 36 weeks old white Leghorn hens, from strain Hi-line W-36, were randomly allocated to 8 dietary treatments and 4 blocks in a completely randomized block design. The experiment lasted for 3 months, starting in the 36th week and ended in the 48week of age. Shell thickness, shell ash percentage, shell percentage, feed consumption, tibial ash and tibial calcium percentage were significantly different (P<0.05) among treatments. Effect of experimental period on shell calcium and phosphorus percentage, egg weight, egg production, feed consumption, egg output was significant (P<0.05). Relative bioavailability of samples ranged from 85 to 105.7%. Overall, samples of Phosphate Iran (F) were considered most effective with regard to relative bioavailability and performance.

The nutritive values of lemon and orange pulps treated with Neurospora sitophila fungus were assessed by chemical composition, in vitro digestibility, in situ (dry matter and nitrogen) degradation methods. The obtained data from the untreated and treated pulps with fungi were compared using t-test. Mean values of the chemical analysis for crude protein, ash, organic mater, NDF and ADF for untreated lemon pulp were 6.3, 6.2, 93.8, 21.3 and 17.9; for treated lemon pulp 25.1, 10.6, 89.4, 12.7 and 6.8; for untreated orange pulp 6.8, 6.5, 94.5, 26.1 and 20.3; and for treated orange pulp 23.2, 8.1, 91.9, 18.5 and 15, respectively. In all cases, a significant difference (P<0.01) between untreated pulp and treated pulp of lemon and orange was observed. Digestibility coefficient of DM, OM and DOMD of untreated lemon pulp were 79.3, 80.5 and 75.5; treated lemon pulp were 91.4, 93.5 and 83.5; untreated orange pulp were 81.5, 82.8 and 78.2%; and treated orange pulp were 91.2, 94.5 and 86.9%, respectively. The digestibility coefficient for the treated pulps (lemon and orange) was significantly (P<0.01) higher than those untreated. The percentages of DM degradability of pulps in nylon bag after 48 hours of incubation with the out-flow rate of 0.05 were as follows: 66.3 for untreated lemon; 75.2 for treated lemon; 68.7 for untreated orange; and 75.5 for treated orange. The DM degradability for the treated pulps (lemon and orange) was significantly (P<0.01) higher than those untreated. The effective degradability of protein after 48 hours of incubation in nylon bag with the out-flow rate of 0.05 was as follows: 12.1 for untreated lemon; 73.7 for treated lemon; 14.8 for untreated orange; and 77.8 for treated orange. The effective degradability of protein for the treated pulps (lemon and orange) was significantly (P<0.01) higher than those untreated. In conclusion, treating citrus pulps with Neurospora sitophila increased the concentration of CP, digestibility coefficients and protein degradability.

Eight hundred and fifty two multiparous Holstein cows in mid-lactation were used in a completely randomized and nested design with two treatments and three replications. Treatments were: 1) Isfahan; and 2) Borkhar-Meymeh. Replications were: 1) class of one to five cows; 2) class of six to ten cows; and 3) class of eleven to twenty cows. Paired t-test was used for the comparison of nutrient consumption and requirement. The obtained results of one-year-old experiment indicated that township and different classes had no effect on consumption of dry matter intake (DMI), net energy lactating (NEL), metabolizable protein (MP), calcium (Ca), phosphorus (P), potassium (K), magnesium (Mg), sodium (Na), chlorine (Cl), sulphur (S), selenium (Se), zinc (Zn), copper (Cu), iodine (I), vitamin A, vitamin D, vitamin E, rumen degradable protein (RDP), neutral detergent fiber (NDF), acid detergent fiber (ADF) and non-fiber carbohydrate (NFC). Township had a significant effect on consumption of rumen undegradable protein (RUP) (P<0/05). Township and different classes had no effect on nutrient requirements of dairy cattle. The difference between consumption and requirement for kg DMI, Mcal/d NEL, g Mp, (g absorbable) Ca, P, K, Mg, Na, Cl, S, mg per kg Se, Zn, Cu, I, Iu/d vitamin A, vitamin D, vitamin E, g/d RDP, RUP, percentage of dry matter NDF, ADF and NFC were -1/6, -2/5, -630/2, -21/8, -5, 40/6, 0, -3/3, 44/2, -7/4, -3/11, -38/7, -3/26, -4/45, -14834, -234, -342/5, -542/5, -886, 28/5, 17/5 and -10/4, respectively. The difference between consumption and requirement was not significant for P, Mg and Na. This difference had a significant effect on the rest of nutrients (P<0/05). The results of this study indicated that we can feed dairy cattle by using diets containing protein supplements and mineral-vitamin premix and improve milk yield and percentage of milk protein's rural areas of Holstein cows.

This study was conducted to assess the effect of culturing oyster mushroom (Pleurotus florida) on the nutritive value of wheat straw. In a completely randomized design, chemical composition and in vitro digestibility of untreated and fungal treated wheat straw were measured by the following treatments: 1) untreated wheat straw (UWS); 2) fermented wheat straw before mushroom fruiting (FTWS); and 3) fermented wheat straw after mushroom harvesting (SPWS), with 4 replicates. In addition, the in vivo digestibility and voluntary intake were determined using 4 cows and 4 sheep and nutritive value index was estimated. The crude protein and in vitro digestibility increased whereas organic matter and cell wall components decreased significantly (P<0.05) in FTWS and SPWS. In vivo digestibility and voluntary intake increased in FTWS when fed to sheep or cow (P<0.05) but no significant differences were found between SPWS and UWS. The digestible dry mater and organic mater intake (g/kg W0.75) were significantly higher (P<0.05) in cow than sheep. The significantly (P<0.05) highest amount of nutritive value index was obtained for FTWS fed to cow and the lowest amount was found for SPWS when fed to sheep. It can be concluded that culturing Pleurotus florida on wheat straw can improve its nutritive value before mushroom fruiting.

This study was undertaken to estimate genetic trend and parameters of Holstein cattle in Khorasan province for milk yield using a spline random regression test day animal model. A total of 32854 monthly test day milk records (twice and thrice a day milking) obtained from 3842 Holstein heifers (progeny of 466 sires) distributed in 125 herds and calved from 2001 to 2005 was used to predict breeding value of individual animals. In the model, fixed effects of herd including year-month of recording, milking times, age at calving (linear and quadratic covariables), Holstein gene percentage (linear covariable) as well as random effects of additive genetic and permanent environment were studied. To take account of the shape of the lactation curve at genetic and environmental levels, cubic spline polynomials were also included in the test day model. Bayesian method by applying Gibbs sampling technique (100000 chains applying RRGIBBS software) was utilized to obtain posterior means of predicted breeding value of animals for milk yield at individual month of lactation. The results showed that mean of breeding value for 305-day milk yield was 52.90 kg (p<0.05). Spearman rank correlations between predicted breeding values at different months of lactation decreased as the interval between them increased. The highest and lowest rank correlations were found between months 8 and 9 (0.998) and between months 1 and 10 (0.312), respectively. Predicted breeding value of 305-day milk had the lowest and highest rank correlations with predicted breeding value at months 1 (0.553) and 6 (0.990), respectively. Regression analysis of average predicted breeding value of progenies in their birth year showed that the amount of genetic trend for 305 day milk yield was 17.75 kg per year, statistically no different from zero (p value=0.165).

This experiment was conducted to determine the performance and intestinal microflora population of broiler chicks in diets supplemented with Roxarsone, Avilamycin and Formycin gold during starter period. A basal diet was formulated according to NRC (1994) recommendation for starter (0-21 d) period and also supplemented with appropriate amounts of the mentioned growth promoters. Each of the treatment was fed to 5 replicates of 18 Ross 308 male broilers. The birds were raised on pen floor for 21 days. The microflora population in crop and ileum parts was measured on appropriate bacteriological media. Results of experiment indicated that supplementation of Roxarsone and Avilamycin, especially their combination to broiler diets, significantly improved body weigh gain of birds (P<0.05). Feed consumption and feed conversion ratios were not affected by any of the mentioned growth promoters. Adding all the growth promoters to the basal diet, except for the treatment of 0.05 percent Formycin gold, decreased total bacterial numbers in crop and ileum parts. Broilers fed diets containing Roxarsone plus Avilamycin had the lowest total bacterial population and were significantly different from those fed the basal diet (P<0.05). Supplementing broiler diets with all the growth promoters increased lactic acid bacteria population in crop, and decreased coliforms population in ileum parts. Lactic acid bacteria population was the highest in broilers fed Roxarsone plus Avilamycin and coliforms population was the lowest in birds fed 0.2 percent Formycin gold, being significantly different from basal diet (P<0.05).

Six hundred young calves, one month to three months old, were used in a completely randomized and nested design with two treatments and three replications. Treatments were: 1) Isfahan; and 2) Borkhar-Meymeh. Replications were: 1) class of one to five cows; 2) class of six to ten cows; and 3) class of eleven to twenty cows. Paired t-test was used for comparison of nutrient consumption and requirement. Results of one-year-old experiment indicated that township and different classes had no effect on consumption of dry matter intake (whole milk and starter), net energy growth (NEg), crude protein (CP), calcium (Ca), phosphorus (P), potassium (K), magnesium (Mg), sodium (Na), chlorine (Cl), sulphur (S), cobalt (Co), copper (Cu), iodine (I), iron (Fe), manganese (Mn), selenium (Se), Zinc (Zn), vitamin A, vitamin D and vitamin E. Treatments (township) had no significant effect on the nutrient requirements of young calves. The daily difference between consumption and requirement for DMI (whole milk Kg/d), DMI (starter Kg/d), NEg Mcal/Kg, CP g/d, percentage of dry matter Ca, P, Mg, Cl, K, Na, S, mg/kg Co, Cu, I, Fe, Mn, Se, IU/kg vitamins A, D and E were - 0/15, -0/092, -0/165, -17/7, -0/09, 0/1, 0/08, 0/53, 0/64, 0, 0/01, -0/1, -7/2, -0/32, -36/9, -14/1, -0/12, 0/5, -4499, -515 and -40/8, respectively. The difference between consumption and requirement had no significant effect on P, Na, S and Zn. This difference had a significant effect on the rest of nutrients (p <0/05). The results of this study indicated that young calves had received low DMI (whole milk and starter), NEg, CP, Ca, Co, Cu, I, Fe, Mn, Se, vit A, vit D and vit E. This shortage can be compensated by increasing DMI (whole milk and starter) protein supplements, vitamin and mineral supplements.

Genetic maps with high genome coverage are becoming increasingly useful in both basic and applied genetic researches. In the last decades, the advent of DNA markers has brought about a magnificent revolution in the production of genetic map, especially in wheat. In the present study, AFLP markers were used to saturate linkage map of 107 doubled haploid individuals produced through Fukuho _Komugi ´ Oligo – Culm crosses received from Japan International Research Center of Agricultural Science (JIRCAS). The framework of genetic map was used as base map for next analysis. AFLP analysis was performed with MseI / PstI as digestive enzymes. The average percentage of polymorphism with AFLP markers was around 16.6%. Data analysis was performed by computer program known as Mapmaker / EXP, Ver. 3.3. In this program, the maximum distance criterion was 50 cM and the minimum LOD equated 3. The drawing of chromosome schema for the linkage groups was performed by Draw map, Ver 1.1. In this analysis, 115 AFLP markers were divided into 10 groups; in addition, some of the markers remained unlinked. The supplementary data analysis along with specific SSR markers identified the chromosome loci of the markers. Ultimately, 71.1% of the markers were assigned to genome A, 16.5% to genome B and only 3% to genome D. The AFLP markers filled 11 gaps in 7 chromosomes (2A, 3A, 7A, 2B, 3B, 5B and 7B). The low coverage of genome D was due to the limited polymorphism and its conservation in different populations. Among the chromosomes, maximum number of markers (60) was assigned to the chromosome 7A. The distribution of the markers on this chromosome was not uniform. Such a distribution was related to the grouping AFLP markers within heterochromatin region, particularly around the centromere.